Deciding mPFC as the target area via c-Fos. mPFC, CA1, CA3, DG and VMH were investigated. mPFC: Higher expression, and larger difference
E-I imbalance: ELISA-Glutamate reduction
Presynaptic
TEM: Reduction of glutamate vesicles
Postsynaptic
Reduced amplitude and decay time of EPSC
Reduced expression of GluN2B and p-GluB2B
Mechanism verification
Recapitualate tests
CamK2aCre;Gpr158flox/flox mice
Rescue tests
Gpr158 re-expression in mPFC with Lenti-virus
Chemogenetic enhancement of CamK2A+ neurons in mPFC